Riboflavin Kinase Protein (RFK) (His tag)
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- Target See all Riboflavin Kinase (RFK) Proteins
- Riboflavin Kinase (RFK)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Riboflavin Kinase protein is labelled with His tag.
- Purpose
- Recombinant Human Flavokinase/RFK Protein (His Tag)
- Sequence
- Met 1-His 155
- Characteristics
- A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product RFK Protein
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 10 % glycerol, pH 8.0
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- Riboflavin Kinase (RFK)
- Alternative Name
- Flavokinase/RFK (RFK Products)
- Synonyms
- RFK Protein, 0610038L10Rik Protein, AF031381 Protein, KOI-4 Protein, RIFK Protein, riboflavin kinase Protein, FMN adenylyltransferase Protein, Riboflavin kinase Protein, RFK Protein, ribF Protein, CNC05850 Protein, Nitsa_1631 Protein, Isop_1070 Protein, Rfk Protein
- Background
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Background: Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
Synonym: RIFK
- Molecular Weight
- 19.5 kDa
- UniProt
- Q969G6
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