PTPN12 Protein

Catalog No. ABIN7317537

Product Details

Target: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purpose: Recombinant Human PTPN12 Protein (Active)

Sequence: Met 1-Gln355

Characteristics: A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site .The specific activity is >20 mol/min/mg

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))

Alternative Name: PTPN12 (PTPN12 Products)

Synonyms: PTP-PEST Protein, PTPG1 Protein, P19-PTP Protein, PTP-P19 Protein, Ptpg1 Protein, RKPTP Protein, protein tyrosine phosphatase, non-receptor type 12 Protein, PTPN12 Protein, Ptpn12 Protein

Background:

Background: PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.

Synonym: PTP-PEST,PTPG1

Molecular Weight: 41.8 kDa