Pleiotrophin Protein (PTN)
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- Target See all Pleiotrophin (PTN) Proteins
- Pleiotrophin (PTN)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purpose
- Recombinant Mouse Pleiotrophin/PTN/HB-GAM Protein (Active)
- Sequence
- Met1-Asp168
- Characteristics
- A DNA sequence encoding the mouse PTN (P63089) (Met1-Asp168) was expressed.
- Purity
- > 95 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized mouse PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc , The EC50 of rat SDC1-Fc is 0.4-1.1 μg/ml.
- Top Product
- Discover our top product PTN Protein
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Pleiotrophin (PTN) (AA 33-168) protein (Fc Tag)
PTN Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Pleiotrophin (PTN) protein (His tag)PTN Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Pleiotrophin (PTN) (AA 33-168) protein (Fc Tag)PTN Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Pleiotrophin (PTN) (AA 45-154) protein (GST tag)PTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pleiotrophin (PTN) (AA 1-168) protein (GST tag)PTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 1M NaCl, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Pleiotrophin (PTN)
- Alternative Name
- Pleiotrophin/PTN/HB-GAM (PTN Products)
- Synonyms
- zHBNF Protein, zgc:111787 Protein, harp Protein, hbnf Protein, X-PTF Protein, hbgf8 Protein, negf1 Protein, ptn Protein, PTN Protein, HARP Protein, HBGF8 Protein, HBNF Protein, NEGF1 Protein, PTN-A Protein, HB-GAM Protein, HBBM Protein, HBGF-8 Protein, OSF-1 Protein, HBBN Protein, OSF Protein, Osf-1 Protein, Osf1 Protein, Hbnf Protein, pleiotrophin Protein, pleiotrophin L homeolog Protein, ptn Protein, PTN Protein, ptn.L Protein, Ptn Protein
- Background
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Background: HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.
Synonym: Pleiotrophin, PTN, Heparin-binding brain mitogen, HBBM, Heparin-binding growth factor 8, HBGF-8, Osteoblast-specific factor 1, OSF-1,HARP,HB-GAM,HBBN,HBNF,OSF,Osf-1,Osf1
- Molecular Weight
- 15.3 kDa
- UniProt
- P63089
- Pathways
- RTK Signaling
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