PAK3 Protein (PAK3) (His tag)

Catalog No. ABIN7317075

Product Details

Target: PAK3

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This PAK3 protein is labelled with His tag.

Purpose: Recombinant Human PAK3 Protein (His Tag)(Active)

Sequence: Met 1-Arg 544

Characteristics: A DNA sequence encoding the human PAK3 isoform 2 (O75914-2) (Met 1-Arg 544) was fused with a polyhistidine tag at the C-terminus.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 98 nmol/min/mg using MBP as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: PAK3

Alternative Name: PAK3 (PAK3 Products)

Synonyms: CDKN1A Protein, MRX30 Protein, MRX47 Protein, OPHN3 Protein, PAK3beta Protein, bPAK Protein, hPAK3 Protein, PAK-3 Protein, Pak65alpha Protein, Pak65beta Protein, Stk4 Protein, beta-PAK Protein, XPak3 Protein, bpak Protein, cdkn1a Protein, mrx30 Protein, mrx47 Protein, ophn3 Protein, pak3 Protein, pak3-A Protein, pak3beta Protein, p21 (RAC1) activated kinase 3 Protein, p21 protein (Cdc42/Rac)-activated kinase 3 Protein, p21 protein (Cdc42/Rac)-activated kinase 3 L homeolog Protein, PAK3 Protein, Pak3 Protein, pak3.L Protein

Background:

Background: PAK3 is a member of PAK proteins, a family of serine/threonine p21-activating kinases, serve as effectors of small Rho GTPases Cdc42 and RAC and have been implicated in a wide range of biological activities. There are six mammalian PAKs which can be divided into two groups: group I PAKs (PAK1-3) and group II PAKs (PAK4-6). Although the two PAK groups are architecturally similar there are differences in their mode of regulation suggesting their cellular functions are likely to be different. Group I p21-activated kinases (PAK1/2/3) is demonstrated as ERK3/ERK4 activation loop kinases. It has been shown that group I PAKs phosphorylate ERK3 and ERK4 on Ser-189 and Ser-186, respectively, both in vitro and in vivo, and that expression of activated Rac1 augments this response. Besides regulation enzymatic activation of ERK3/ERK4, PAKs can also play roles in downstream activation of MAP kinase-activated protein kinase 5 (MK5) in vivo. Thus, the  group I PAKs act as upstream activators of ERK3 and ERK4 and unravel a novel PAK-ERK3/ERK4-MK5 signaling pathway. In clinical, PAK has been proposed as a potential therapeutic target in schwannomas.

Synonym: bPAK;CDKN1A;hPAK3;MRX30;MRX47;OPHN3;PAK3beta

Molecular Weight: 62 kDa