Notch1 Protein (His tag)

Catalog No. ABIN7320796

Product Details

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This Notch1 protein is labelled with His tag.

Purpose: Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)

Sequence: Ala18-Gln526

Characteristics: Recombinant Mouse Notch 1 is produced by our Mammalian expression system and the target gene encoding Ala18-Gln526 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Motch A/NOTCH1 (NOTCH1 Products)

Synonyms: notch1 Protein, NOTCH1 Protein, hn1 Protein, tan1 Protein, notch Protein, xotch Protein, xnotch Protein, notch-1 Protein, xnotch1 Protein, TAN1 Protein, hN1 Protein, 9930111A19Rik Protein, Mis6 Protein, N1 Protein, Tan1 Protein, lin-12 Protein, NOTCH Protein, Xotch Protein, notch1-a Protein, notch 1 Protein, fibropellin-1 Protein, Notch homolog 1, translocation-associated (Drosophila) Protein, Notch homolog 1, translocation-associated Protein, notch 1 S homeolog Protein, notch1 Protein, LOC100631526 Protein, NOTCH1 Protein, Notch1 Protein, notch1.S Protein

Background:

Background: Mouse Notch1 is a 300 kDa type I transmembrane glycoprotein and it functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Mouse Notch1 is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1707 aa extracellular domain (ECD) with 36 EGFlike repeats and three Lin12/notch repeats, a 21 aa transmembrane segment and a 785 aa cytoplasmic domain that contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. Notch1 may play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation and may be involved in mesoderm development, somite formation and neurogenesis.

Synonym: Neurogenic locus notch homolog protein 1, Notch 1, Motch A, Mt14,lin-12,Mis6,N1,Tan1

Molecular Weight: 54.4 kDa

UniProt: Q01705

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development