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NOG Protein (Fc Tag)

NOG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Catalog No. ABIN7317939
  • Target See all NOG Proteins
    NOG (Noggin (NOG))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 11
    • 10
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Source
    • 17
    • 7
    • 4
    • 3
    • 3
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This NOG protein is labelled with Fc Tag.
    Purpose
    Recombinant Human Noggin/NOG Protein (aa 1-232, Fc Tag)(Active)
    Sequence
    Met 1-Cys 232
    Characteristics
    A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with the Fc region of human IgG1 at the C-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Endotoxin Level
    < 1.0 EU per μg as determined by the LAL method.
    Biological Activity Comment
    1. Measured by its ability to inhibit BMP2-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1.5-2.0 μg /mL in the presence of 0.25-0.5 μg/mL of BMP-2.2. Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 50 ng/mL of hBMP4.
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  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    NOG (Noggin (NOG))
    Alternative Name
    Noggin/NOG (NOG Products)
    Background

    Background: Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.

    Synonym: Noggin;SYM1;SYNS1

    Molecular Weight
    49.8 kDa
    NCBI Accession
    NP_005441
    Pathways
    Stem Cell Maintenance, Tube Formation
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