Nicastrin Protein (NCSTN) (His tag)

Catalog No. ABIN7320108

Product Details

Target: Nicastrin (NCSTN)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Nicastrin protein is labelled with His tag.

Purpose: Recombinant Mouse Nicastrin/NCSTN Protein (His Tag)

Sequence: Met1-Gln668

Characteristics: A DNA sequence encoding the mouse NCSTN (P57716) (Met1-Gln668) was expressed with a C-terminal polyhistidine tag.

Purity: > 99 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Nicastrin (NCSTN)

Alternative Name: Nicastrin/NCSTN (NCSTN Products)

Background:

Background: Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.

Synonym: 9430068N19Rik,AA727311,Aph2,D1Dau13e,Kiaa0253,mKIAA0253,NCSTN,Nct

Molecular Weight: 73 kDa

UniProt: P57716

Pathways: Notch Signaling, Neurotrophin Signaling Pathway