Nicastrin Protein (NCSTN) (His tag)

Catalog No. ABIN7197196

Product Details

Target: Nicastrin (NCSTN)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Nicastrin protein is labelled with His tag.

Purpose: Recombinant Human Nicastrin/NCSTN Protein (His Tag)

Sequence: Met 1-Glu 669

Characteristics: A DNA sequence encoding the human NCSTN (NP_056146.1) extracellular domain (Met 1-Glu 669) was expressed, with a polyhistidine tag at the C-terminus.

Purity: > 93 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Nicastrin (NCSTN)

Alternative Name: Nicastrin/NCSTN (NCSTN Products)

Synonyms: CG7012 Protein, Dmel\\CG7012 Protein, Nct Protein, Nic Protein, S5A Protein, ST96B Protein, agro Protein, dNct Protein, nic Protein, NCSTN Protein, DKFZp469O2418 Protein, APH2 Protein, 9430068N19Rik Protein, AA727311 Protein, Aph2 Protein, D1Dau13e Protein, Kiaa0253 Protein, mKIAA0253 Protein, nicastrin Protein, Nicastrin Protein, NCSTN Protein, nct Protein, ncstn Protein, CpipJ_CPIJ001617 Protein, Ncstn Protein, aph-2 Protein

Background:

Background: Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.

Synonym: APH2,ATAG1874,KIAA0253,NCSTN,RP11-517F10.1

Molecular Weight: 72.4 kDa

NCBI Accession: NP_056146

Pathways: Notch Signaling, Neurotrophin Signaling Pathway