NEDD8 Protein
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- Target See all NEDD8 Proteins
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human NEDD8
- Sequence
- Met1-Gly76
- Characteristics
- Recombinant Human Neural precursor cell expressed developmentally down-regulated protein 8 is produced by our E.coli expression system and the target gene encoding Met1-Gly76 is expressed.
- Purity
- >95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) protein (GST tag)
NEDD8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, pH 8.0.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- Alternative Name
- NEDD8 (NEDD8 Products)
- Synonyms
- NEDD-8 Protein, Rub1 Protein, CG10679 Protein, Dmel\\CG10679 Protein, NEDD8 Protein, dNEDD8 Protein, nedd8 Protein, ubiquitin Protein, si:zc14a17.5 Protein, GB17785 Protein, DDBDRAFT_0218116 Protein, DDBDRAFT_0238041 Protein, DDB_0218116 Protein, DDB_0238041 Protein, nedd8.S Protein, neural precursor cell expressed, developmentally down-regulated 8 Protein, neural precursor cell expressed, developmentally down-regulated gene 8 Protein, CG10679 gene product from transcript CG10679-RB Protein, Nedd8 Protein, ubiquitin-like protein Nedd8 Protein, neural precursor cell expressed, developmentally down-regulated 8 L homeolog Protein, NEDD8 Protein, Nedd8 Protein, nedd8 Protein, nedd8.L Protein
- Background
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Background: Human NEDD8 shares 60 % amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Synonym: Neural precursor cell expressed developmentally down-regulated protein 8, NEDD8, Neddylin, Ubiquitin-like protein Nedd8
- Molecular Weight
- 8.6 kDa
- UniProt
- Q15843
- Pathways
- Ubiquitin Proteasome Pathway
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