MAP4K2 Protein (GST tag,His tag)

Catalog No. ABIN7317061

Product Details

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MAP4K2 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human MAP4K2/GC Kinase Protein (His & GST Tag)

Sequence: Met 1-Tyr 812

Characteristics: A DNA sequence encoding the human MAP4K2 (AAH47865.1) (Met 1-Tyr 812) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 87 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Alternative Name: MAP4K2/GC Kinase (MAP4K2 Products)

Synonyms: BL44 Protein, GCK Protein, RAB8IP Protein, AI385662 Protein, Rab8ip Protein, MAP4K2 Protein, dZ150L22.2 Protein, map4k2l Protein, map4k2l-2 Protein, map4k3l Protein, si:dz150l22.2 Protein, si:dz263j20.1 Protein, zgc:136670 Protein, mitogen-activated protein kinase kinase kinase kinase 2 Protein, mitogen activated protein kinase kinase kinase kinase 2 Protein, MAP4K2 Protein, Map4k2 Protein, LOC103346779 Protein, map4k2 Protein

Background:

Background: Mitogen-activated protein kinase kinase kinase kinase 2, also known as B lymphocyte serine/threonine-protein kinase, Germinal center kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, Rab8-interacting protein and MAP4K2, is a cytoplasm and peripheral membrane protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K2 contains one CNH domain and one protein kinase domain. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. MAP4K2 can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. It is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1 / MEKK1. MAP4K2 enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. It may also play a role in the regulation of vesicle targeting or fusion.

Synonym: BL44;GCK;MAP4K2;RAB8IP

Molecular Weight: 119 kDa