c-MET Protein (Fc Tag)

Catalog No. ABIN7318575

Product Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This c-MET protein is labelled with Fc Tag.

Purpose: Recombinant Human HGFR/c-MET Protein (Fc Tag)(Active)

Sequence: Glu25-Thr932

Characteristics: Recombinant Human Hepatocyte Growth Factor Receptor is produced by our Mammalian expression system and the target gene encoding Glu25-Thr932 is expressed with a Fc tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized HGF R-Fc at 2μg/ml(100 μl/well) can bind Human HGF-His(Cat: PKSH032538). The ED50 of HGF R-Fc is 0.82 ug/mL

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: HGFR/c-MET (MET Products)

Synonyms: AUTS9 Protein, HGFR Protein, RCCP2 Protein, c-Met Protein, AI838057 Protein, HGF Protein, Par4 Protein, Hgfr Protein, c-met Protein, MET Protein, C-MET Protein, met Protein, met-A Protein, MET proto-oncogene, receptor tyrosine kinase Protein, met proto-oncogene Protein, MET proto-oncogene, receptor tyrosine kinase L homeolog Protein, MET Protein, Met Protein, met.L Protein

Background:

Background: Hepatocyte growth factor receptor (HGF R) is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. Mature HGF R is a disulfide-linked dimer composed of a 50 kDa extracellular α chain and a 145 kDa transmembrane β chain. Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the α and β chains. The sema domain, which is formed by both α and β chains of HGF R, mediates both ligand binding and receptor dimerization. HGF stimulation induces HGF R downregulation via internalization and proteasomedependent degradation. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells.

Synonym: Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET

Molecular Weight: 128.4 kDa

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor