MMP12 Protein
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- Target See all MMP12 Proteins
- MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human MMP12/MMP-12/HME Protein (catalytic domain)(Active)
- Sequence
- Gly106-Asn268
- Characteristics
- A DNA sequence encoding human MMP12 (NP_002417.2) (Gly106-Asn268) was expressed with a N-terminal Met.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 800 pmoles/min/g
- Top Product
- Discover our top product MMP12 Protein
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) (AA 292-401) protein (GST tag)
MMP12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) (AA 1-470) protein (GST tag)MMP12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) protein (His tag)MMP12 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 10 mM Hepes, 2 mM CaCl2, 250 mM NaCl, pH 7.0
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
- Alternative Name
- MMP12/MMP-12/HME (MMP12 Products)
- Synonyms
- MMP12 Protein, AV378681 Protein, Mmel Protein, Mme Protein, HME Protein, ME Protein, MME Protein, MMP-12 Protein, matrix metallopeptidase 12 Protein, MMP12 Protein, Mmp12 Protein
- Background
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Background: Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Macrophage metalloelastase, also known as Matrix metalloproteinase-12, Macrophage elastase, MMP12, and MMP-12, is a secreted protein which belongs to the peptidase M10A family. MMP12 is a macrophage-secreted elastase that is highly induced in the liver and lung in response to S. mansoni eggs and contains four hemopexin-like domains. MMP12 is a proteolytic enzyme responsible for cleavage of plasminogen to angiotensin, which has an angiostatic effect. It may be involved in tissue injury and remodeling and has significant elastolytic activity. It may be related to prognosis in breast cancer patients. MMP12 promotes fibrosis by limiting the expression of specific ECM-degrading MMPs. Like MMP12, MMP13 expression is highly dependent on IL-13 and type I I-IL-4 receptor signaling. MMP12 is a potent proinflammatory and oncogenic molecule. MMP12 up-regulation plays a critical role in emphysema to lung cancer transition that is facilitated by inflammation.
Synonym: HME;ME;MME;MMP-12
- Molecular Weight
- 18.2 kDa
- NCBI Accession
- NP_002417
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