KARS Protein (His tag)
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- Target See all KARS Proteins
- KARS (Lysyl-tRNA Synthetase (KARS))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This KARS protein is labelled with His tag.
- Purpose
- Recombinant Human KARS Protein (His Tag)
- Sequence
- Ala2-Val597
- Characteristics
- Recombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product KARS Protein
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Lysyl-tRNA Synthetase (KARS) (AA 1-597) protein (GST tag)
KARS Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Lysyl-tRNA Synthetase (KARS) (AA 63-597) protein (His tag)KARS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Lysyl-tRNA Synthetase (KARS) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)KARS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM DTT,20 % glycerol, pH 8.0.
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- KARS (Lysyl-tRNA Synthetase (KARS))
- Alternative Name
- KARS (KARS Products)
- Synonyms
- CG12141 Protein, Dmel\\CG12141 Protein, KRS Protein, LysRS Protein, cb530 Protein, wu:fa16h02 Protein, zgc:92483 Protein, kars Protein, MGC53345 Protein, kars2 Protein, krs Protein, krs-1 Protein, KARS Protein, Tb06.28P18.190 Protein, CMTRIB Protein, DFNB89 Protein, KARS1 Protein, KARS2 Protein, AA589550 Protein, AL024334 Protein, AL033315 Protein, AL033367 Protein, D8Ertd698e Protein, D8Wsu108e Protein, mKIAA0070 Protein, Lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase S homeolog Protein, lysyl-tRNA synthetase L homeolog Protein, LysRS Protein, kars Protein, kars.S Protein, kars.L Protein, Bm1_16500 Protein, lysS Protein, Taci_1118 Protein, Tpau_1737 Protein, Arch_0261 Protein, Trad_2960 Protein, Spirs_3699 Protein, KARS Protein, LOC100280510 Protein, Tb927.6.1510 Protein, Kars Protein
- Background
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Background: Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).
Synonym: Lysine--tRNA Ligase, Lysyl-tRNA Synthetase, LysRS, KARS, KIAA0070
- Molecular Weight
- 69.1 kDa
- UniProt
- Q15046
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