Leptin Protein (LEP)

Catalog No. ABIN7318719

Product Details

Target: Leptin (LEP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Leptin Protein (Active)

Sequence: Val22-Cys167

Characteristics: Recombinant Human Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Leptin at 10μg/ml(100 μl/well) can bind Human CD295-His(Cat: PKSH033606).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Leptin (LEP)

Alternative Name: Leptin (LEP Products)

Synonyms: ob Protein, obese Protein, LEPD Protein, OB Protein, OBS Protein, leptin Protein, Lep Protein, LEP Protein, lep Protein

Background:

Background: Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level. When mutations are introduced in obese gene, mice with impaired function of leptin are massively obese and in high risk of diabetes. Leptin deficiency reduces metablic rate. Leptin deficient mice are less active and with lower body temperature than normal animals. Human Leptin shares approximately 84 % sequence identity with the mouse protein. Human Leptin consists of 167 amino acid residue including a 21 amino acid residue signal sequence and 146 amino acid residue mature protein sequence.

Synonym: Leptin, Obese Protein, Obesity Factor, LEP, OB, OBS

Molecular Weight: 16.1 kDa

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process