Layilin Protein (LAYN) (His tag)

Catalog No. ABIN7321084

Product Details

Target: Layilin (LAYN)

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This Layilin protein is labelled with His tag.

Purpose: Recombinant Rat Layilin/LAYN Protein (His Tag)

Sequence: Met1-Glu224

Characteristics: A DNA sequence encoding the rat LAYN (NP_001178926.1) (Met1-Glu224) was expressed with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Layilin (LAYN)

Alternative Name: Layilin/LAYN (LAYN Products)

Synonyms: LAYN Protein, si:dkey-27p23.2 Protein, E030012M19Rik Protein, Gm511 Protein, RGD1564444 Protein, layilin Protein, layilin a Protein, LAYN Protein, layna Protein, Layn Protein

Background:

Background: Layilin, a recently characterized as a 55 kDa transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, a ten-amino acid motif in the layilin cytoplasmic domain is sufficient for talin binding, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Furthermore, talin binds layilin, PIPK1gamma and integrins in similar although subtly different ways. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. The research suggests that layilin may mediate signals from extracellular matrix to the cell cytoskeleton via interaction with different intracellular binding partners and thereby be involved in the modulation of cortical structures in the cell. All the above actions reveal an interesting parallel between layilin and the known HA receptor CD44. In addition, merlin and radixin have been identified as different intracellular binding partners of layilin. Accordingly, it has been suggested that layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction. In addition, layilin might play an important role in the process of invasion and lymphatic metastasis of lung carcinoma.

Synonym: LAYN

Molecular Weight: 24.3 kDa

NCBI Accession: NP_001178926