Latexin Protein (LXN) (His tag)

Catalog No. ABIN7196737

Product Details

Target: Latexin (LXN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Latexin protein is labelled with His tag.

Purpose: Recombinant Mouse Latexin/LXN Protein (His Tag)(Active)

Sequence: Glu 2-Glu 222

Characteristics: A DNA sequence encoding the mouse LXN (NP_058033.2) (Glu 2-Glu 222) was expressed, with a polyhistide tag at the N-terminus.

Purity: > 95 % as determined by SDS-PAGE

Biological Activity Comment: Measured by its ability to inhibit carboxypeptidase-A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis(2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al, 1999, J. Biol. Chem. 274:30468). The IC50 value is <2.0 nM .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Latexin (LXN)

Alternative Name: Latexin/LXN (LXN Products)

Synonyms: wu:fk66c02 Protein, zgc:103612 Protein, LXN Protein, lxn Protein, ECI Protein, TCI Protein, latexin Protein, lxn Protein, LXN Protein, Lxn Protein

Background:

Background: Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.

Synonym: MGC144352, MGC144353

Molecular Weight: 26.3 kDa

NCBI Accession: NP_058033