KEAP1 Protein
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- Target See all KEAP1 Proteins
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purpose
- Recombinant Human KEAP1/INRF2 Protein
- Sequence
- Gln2-Cys624
- Characteristics
- A DNA sequence encoding the human KEAP1 (Q14145) (Gln2-Cys624) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product KEAP1 Protein
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Kelch-Like ECH-Associated Protein 1 (KEAP1) (AA 1-624) protein (GST tag)
KEAP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Kelch-Like ECH-Associated Protein 1 (KEAP1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)KEAP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Kelch-Like ECH-Associated Protein 1 (KEAP1) (AA 378-486) protein (His-IF2DI Tag)KEAP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10 % glycerol, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
- Alternative Name
- KEAP1/INRF2 (KEAP1 Products)
- Synonyms
- INrf2 Protein, KLHL19 Protein, INRF2 Protein, mKIAA0132 Protein, Inrf2 Protein, fb36f11 Protein, keap1 Protein, wu:fb36f11 Protein, KEAP1 Protein, klhl19 Protein, zgc:171654 Protein, kelch like ECH associated protein 1 Protein, kelch-like ECH-associated protein 1 Protein, Kelch-like ECH-associated protein 1 Protein, kelch-like ECH-associated protein 1a Protein, kelch like ECH associated protein 1 L homeolog Protein, kelch-like ECH-associated protein 1b Protein, KEAP1 Protein, Keap1 Protein, keap1a Protein, keap1 Protein, keap1.L Protein, keap1b Protein
- Background
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Background: Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
Synonym: INRF2,KEAP-1,KLHL19
- Molecular Weight
- 69.7 kDa
- UniProt
- Q14145
- Pathways
- Maintenance of Protein Location
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