IL36A/IL1F6 Protein

Catalog No. ABIN7319787

Product Details

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Interleukin-36 alpha/IL-36 alpha

Sequence: Lys6-Phe158

Characteristics: Recombinant Human Interleukin-36 Alpha is produced by our E.coli expression system and the target gene encoding Lys6-Phe158 is expressed.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using A-431 Human epithelial carcinoma cells. The ED50 for this effect is 12.0 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 0.02 % Tween 20, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Alternative Name: Interleukin-36 alpha/IL-36 alpha (IL1F6 Products)

Synonyms: Il1f6 Protein, Fil1 Protein, IL-1H1 Protein, IL1RP2 Protein, Il1f9 Protein, Il36a Protein, FIL1 Protein, FIL1(EPSILON) Protein, FIL1E Protein, IL-1F6 Protein, IL1(EPSILON) Protein, IL1F6 Protein, interleukin 36, alpha Protein, interleukin 1 family, member 6 (epsilon) Protein, interleukin 1 family, member 6 Protein, interleukin 36 alpha Protein, Il36a Protein, IL1F6 Protein, IL36A Protein, Il1f6 Protein

Background:

Background: Human Interleukin-36α (IL-36α) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

Synonym: Interleukin-36 Alpha, FIL1 Epsilon, Interleukin-1 Epsilon, IL-1 Epsilon, Interleukin-1 Family Member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Molecular Weight: 17.1 kDa

UniProt: Q9UHA7

Pathways: Cancer Immune Checkpoints