IGF2 Protein

Catalog No. ABIN7318628

Product Details

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human IGF-II/IGF2 Protein (Active)

Sequence: Ala25-Glu91

Characteristics: Recombinant Human Long Insulin-Like Growth Factor II is produced by our E.coli expression system and the target gene encoding Ala25-Glu91 is expressed.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 15.7 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 5 mM Hac, pH ~3.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Alternative Name: IGF-II/IGF2 (IGF2 Products)

Background:

Background: Insulin-Like Growth Factor II (IGF2) belongs to the insulin family of polypeptide growth factors that is involved in development and growth. Members of this family are structurally homologous to proinsulin, and share higher sequence identity. IGF2 is expressed only from the paternally inherited allele and believed to be secreted by the liver and to circulate in the blood. IGF2 possess growth-promoting activity and can stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF2 is influenced by placental lactogen and may play a role in fetal development.

Synonym: Insulin-Like Growth Factor II, IGF-II, Somatomedin-A, IGF2, PP1446

Molecular Weight: 8.9 kDa

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation