HMGB1 Protein (Fc Tag)
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- Target See all HMGB1 Proteins
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HMGB1 protein is labelled with Fc Tag.
- Purpose
- Recombinant Mouse HMGB1/HMG1 Protein (Fc Tag)(Active)
- Sequence
- Met 1-Glu 215
- Characteristics
- A DNA sequence encoding the mouse HMGB1 (P63158) (Met 1-Glu 215) was fused with the Fc region of human IgG1 at the N-terminus.
- Purity
- > 85 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to bind mouse AGER-His in functional ELISA.
- Top Product
- Discover our top product HMGB1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Alternative Name
- HMGB1/HMG1 (HMGB1 Products)
- Synonyms
- HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein
- Background
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Background: High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis is present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal "necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. In addition, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation.
Synonym: amphoterin;DEF;HMG-1;Hmg1;p30;SBP-1
- Molecular Weight
- 53.3 kDa
- UniProt
- P63158
- Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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