GDNF Protein

Catalog No. ABIN7318534

Product Details

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human GDNF Protein (Active)

Sequence: Ser78-Ile211

Characteristics: Recombinant Human Glial Cell Line-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ser78-Ile211 is expressed.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human GDNF at 2μg/ml(100 μl/well) can bind Human GFRA1-His(Cat: PKSH033670).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.25.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF (GDNF Products)

Synonyms: gdnf Protein, GDNF Protein, AI385739 Protein, gdnfa Protein, hfb1-gdnf Protein, ATF1 Protein, ATF2 Protein, HFB1-GDNF Protein, HSCR3 Protein, glial cell derived neurotrophic factor Protein, glial cell derived neurotrophic factor a Protein, glial cell line derived neurotrophic factor Protein, glial cell derived neurotrophic factor L homeolog Protein, Gdnf Protein, gdnfa Protein, GDNF Protein, gdnf.L Protein

Background:

Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-β superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.

Synonym: Glial Cell Line-Derived Neurotrophic Factor, hGDNF, Astrocyte-Derived Trophic Factor, ATF, GDNF

Molecular Weight: 15.1 kDa

UniProt: P39905

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration