FCN1 Protein (His tag)
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- Target See all FCN1 Proteins
- FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FCN1 protein is labelled with His tag.
- Purpose
- Recombinant Human Ficolin-1/Ficolin-A/FCN1 Protein (His Tag)(Active)
- Sequence
- Met 1-Ala 326
- Characteristics
- A DNA sequence encoding the human FCN1 (NP_001994.2) precursor (Met 1-Ala 326) was expressed with a C-terminal polyhistidine tag.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA.1. Immobilized FCN1 at 10 μg/ml can bind biotinylated recombinant human Fetuin-A with a linear range of 16-2000 ng/ml.2. Immobilized FCN1 at 10 μg/ml can bind biotinylated recombinant mouse Fetuin-A with a linear range of 16-2000 ng/ml.
- Top Product
- Discover our top product FCN1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))
- Alternative Name
- Ficolin-1/Ficolin-A/FCN1 (FCN1 Products)
- Synonyms
- FCNM Protein, FCNB Protein, FCN2 Protein, FCN1 Protein, ficolin 1 Protein, ficolin (collagen/fibrinogen domain containing) 1 Protein, ficolin-1 Protein, FCN1 Protein, Fcn1 Protein, LOC100069029 Protein
- Background
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Background: Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. The Ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. Three Ficolins have been identified in humans: L-Ficolin, H-Ficolin and M-Ficolin (also referred to as Ficolin-2, -3 and -1, respectively). They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Dysfunction or abnormal expressions of Ficolins may involved in the pathogenesis of human diseases including infectious and inflammatory diseases, autoimmune disease and clinical syndrome of preeclampsia. They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Upon recognition of the infectious agent, the Ficolins act through two distinct routes: initiate the lectin pathway of complement activation through attached serine proteases (MASPs), and a primitive opsonophagocytosis thus limiting the infection and concurrently orchestrating the subsequent adaptive clonal immune response. Ficolin-1 (FCN1) is predominantly expressed in the peripheral blood leukocytes.
Synonym: FCN1,FCNA,FCNM
- Molecular Weight
- 33.6 kDa
- NCBI Accession
- NP_001994
- Pathways
- Complement System
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