Fibronectin Protein
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- Target See all Fibronectin Proteins
- Fibronectin
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human Fibronectin/FN Protein (Active)
- Sequence
- Pro1270-Ser1546&Ala1721-Thr2016
- Characteristics
- Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
- Top Product
- Discover our top product Fibronectin Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25 % Sucrose, pH 6.2.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Fibronectin
- Alternative Name
- Fibronectin/FN (Fibronectin Products)
- Synonyms
- FN Protein, cig Protein, fibronectin Protein, finc Protein, lets Protein, msf Protein, CIG Protein, ED-B Protein, FINC Protein, FNZ Protein, GFND Protein, GFND2 Protein, LETS Protein, MSF Protein, E330027I09 Protein, Fn Protein, Fn-1 Protein, FIBNEC Protein, fn-1 Protein, fibronectin 1 Protein, FN1 Protein, fn1 Protein, Fn1 Protein
- Background
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Background: Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
Synonym: Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
- Molecular Weight
- 62.7 kDa
- UniProt
- P02751
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