ECH1 Protein

Catalog No. ABIN7319227

Product Details

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human ECH1 Protein

Sequence: Thr34-Leu328

Characteristics: Recombinant Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial is produced by our E.coli expression system and the target gene encoding Thr34-Leu328 is expressed with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,100 mM NaCl,10 % Glycerol, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Alternative Name: ECH1 (ECH1 Products)

Synonyms: HPXEL Protein, Pxel Protein, AA617331 Protein, enoyl-CoA hydratase 1, peroxisomal Protein, enoyl CoA hydratase 1, peroxisomal Protein, enoyl-CoA hydratase 1 Protein, delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Protein, enoyl-CoA hydratase 1, peroxisomal L homeolog Protein, enoyl coenzyme A hydratase 1, peroxisomal Protein, ech1 Protein, ECH1 Protein, LOC747511 Protein, ech1.L Protein, Ech1 Protein

Background:

Background: Human delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase(ECH1) is a member of the hydratase/isomerase superfamily and contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The rat orthologlocalizes to the matrix of both the peroxisome and mitochondria.It can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 plays an important role in the auxiliary step of the fatty acid beta-oxidation pathway.

Synonym: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial,ECH1,

Molecular Weight: 34.5 kDa

UniProt: Q13011

Pathways: Monocarboxylic Acid Catabolic Process