ENO1 Protein (His tag)

Catalog No. ABIN7319760

Product Details

Target: ENO1 (Enolase 1 (ENO1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ENO1 protein is labelled with His tag.

Purpose: Recombinant Human Alpha-enolase/Enolase 1/MPB-1/NNE(C-6His)

Sequence: Met1-Lys434

Characteristics: Recombinant Human Alpha-enolase is produced by our E.coli expression system and the target gene encoding Met1-Lys434 is expressed with a 6His tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, 1 mM MgSO4, 20 % Glycerol, pH 7.5 .

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: Alpha-enolase/Enolase 1/MPB-1/NNE (ENO1 Products)

Synonyms: enolase 1 Protein, F2P9.10 Protein, F2P9_10 Protein, Pgh1 Protein, pENO1 Protein, enolase 1 Protein, phosphopyruvate hydratase Protein, ENO1 Protein, eno1 Protein

Background: Alpha-enolase, also known as MPB-1, NNE, Enolase 1, belongs to the enolase family. Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. Isoform MBP-1 interacts with TRAPPC2B. ENO1 interacts with ENO4 and PGAM2. The alpha/alpha homodimer of ENO1 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses and may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. ENO1 also stimulates immunoglobulin production.

Molecular Weight: 48 kDa

UniProt: P06733