DOT1L Protein

Catalog No. ABIN7317542

Product Details

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human DOT1L/KMT4 Protein

Sequence: Gly 2-Lys 416

Characteristics: A DNA sequence encoding the human DOT1L (NP_115871.1) N-terminal segment (Gly 2-Lys 416) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM HEPES, 150 mM NaCl, 1 mM EDTA, 15 % glycerol, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L/KMT4 (DOT1L Products)

Synonyms: NV11307 Protein, DOT1L Protein, DOT1 Protein, KMT4 Protein, A630076O07 Protein, AW907654 Protein, Dot1 Protein, mDot1 Protein, histone-lysine N-methyltransferase, H3 lysine-79 specific Protein, DOT1 like histone lysine methyltransferase Protein, histone methyltransferase Protein, DOT1-like, histone H3 methyltransferase (S. cerevisiae) Protein, LOC100116648 Protein, DOT1L Protein, DOT1 Protein, Dot1l Protein

Background:

Background: Histone-lysine N-methyltransferase, H3 lysine-79 specific, also known as Histone H3-K79 methyltransferase, DOT1-like protein, Lysine N-methyltransferase 4 and DOT1L, is a nucleus protein which belongs to the DOT1 family. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. Methylation of lysine 79 on histone H3 (H3K79) is mediated by DOT1L. It is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription. Mass spectrometry of the DOT1L-containing complex revealed that AF9, ENL and NPM1 were shown to be major DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner.

Synonym: DOT1;KMT4

Molecular Weight: 47.6 kDa

NCBI Accession: NP_115871