DDOST Protein

Catalog No. ABIN7195445

Product Details

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human DDOST/OST48 Protein

Sequence: Ser 43-Pro 427

Characteristics: A DNA sequence encoding the human DDOST (P39656-1) extracellular domain (Ser 43-Pro 427) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: DDOST/OST48 (DDOST Products)

Synonyms: AGER1 Protein, CDG1R Protein, OKSWcl45 Protein, OST Protein, OST48 Protein, WBP1 Protein, DDOST Protein, wu:fa11d01 Protein, zgc:66068 Protein, zgc:77478 Protein, Ddost Protein, CG9022 Protein, DmOST50 Protein, Dmel\\CG9022 Protein, DrOST Protein, OST48/WBP1 Protein, Ots48 Protein, anon-EST:Liang-1.11 Protein, clone 1.11 Protein, ost48 Protein, dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Protein, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) S homeolog Protein, dolichyl-diphosphooligosaccharide-protein glycosyltransferase Protein, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) Protein, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Protein, dolichyl-di-phosphooligosaccharide-protein glycotransferase Protein, Oligosaccharyltransferase 48kD subunit Protein, DDOST Protein, ddost.S Protein, GTR26 Protein, ddost Protein, Ddost Protein, LOC5580056 Protein, PTRG_01946 Protein, Ost48 Protein

Background:

Background: The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase) (DDOST), or 48- kDa subunit (OST48) is one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric complex composed of three type I transmembrane proteins of the endoplasmic reticulum: ribophorin I (RI), ribophorin II (RII), and OST48. OST48 is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum (ER). OST48 does not show significant amino acid sequence homology to either ribophorin I or II. It had been found that only the luminal domain of RI contains ER retention information. The dilysine motif in OST48 functions as an ER localization motif because OST48 in which the two lysine residues are replaced by serine (OST48ss) is no longer retained in the ER and is found instead also at the plasma membrane.

Synonym: AGER1,CDG1R,OKSWcl45,OST,OST48,WBP1

Molecular Weight: 42.7 kDa

Pathways: Cell RedoxHomeostasis