CLMP Protein (His tag)
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- Target See all CLMP Proteins
- CLMP (CXADR-Like Membrane Protein (CLMP))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Rat
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CLMP protein is labelled with His tag.
- Purpose
- Recombinant Rat ASAM Protein (His Tag)(Active)
- Sequence
- Met1-Met232
- Characteristics
- A DNA sequence encoding the rat ASAM (Q8K1G0) (Met1-Met232) was expressed, fused with a polyhistidine tag at the C-terminus.
- Purity
- > 85 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 10E4 cells/well are added to Recombinant Rat ASAM coated plates (12.5 μg/mL with 100 μL/well), 35-70% cells will adhere after 1 hour incubation at 37°C.
- Top Product
- Discover our top product CLMP Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CLMP (CXADR-Like Membrane Protein (CLMP))
- Alternative Name
- ASAM (CLMP Products)
- Synonyms
- ACAM Protein, ASAM Protein, CSBM Protein, CSBS Protein, Asam Protein, Ol16 Protein, 9030425E11Rik Protein, ASP5 Protein, AW557819 Protein, CXADR like membrane protein Protein, CXADR-like membrane protein Protein, CLMP Protein, Clmp Protein
- Background
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Background: Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.
Synonym: ASAM,Clmp ,Acam
- Molecular Weight
- 25.6 kDa
- UniProt
- Q8K1G0
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