COCH Protein (His tag)

Catalog No. ABIN7317572

Product Details

Target: COCH (Cochlin (COCH))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This COCH protein is labelled with His tag.

Purpose: Recombinant Human Cochlin/COCH Protein (His Tag)

Sequence: Glu 25-Gln 550

Characteristics: A DNA sequence encoding the mature form of human COCH (NP_001128530.1) (Glu 25-Gln 550) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: COCH (Cochlin (COCH))

Alternative Name: Cochlin/COCH (COCH Products)

Synonyms: AW122937 Protein, Coch-5B2 Protein, D12H14S564E Protein, COCH-5B2 Protein, COCH5B2 Protein, DFNA9 Protein, cochlin Protein, Coch Protein, COCH Protein

Background:

Background: Cochlin, also known as COCH-5B2 and COCH, is a secreted protein which contains one LCCL domain and two VWFA domains. It is an abundant inner ear protein expressed as multiple isoforms. Its function is also unknown, but it is suspected to be an extracellular matrix component. Cochlin and type II collagen are major constituents of the inner ear extracellular matrix, and Cochlin constitutes 70 % of non-collagenous protein in the inner ear, the cochlin isoforms can be classified into three subgroups, p63s, p44s and p40s. The expression of cochlin is highly specific to the inner ear. Eleven missense mutation and one in-frame deletion have been reported in the COCH gene, causing hereditary progressive sensorineural hearing loss and vestibular dysfunction, deafness autosomal dominant type 9 (DFNA9). The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles. Defects in COCH may contribute to Meniere disease which is an autosomal dominant disorder characterized by hearing loss associated with episodic vertigo.

Synonym: COCH-5B2,COCH5B2,DFNA9

Molecular Weight: 59.4 kDa

NCBI Accession: NP_001128530

Pathways: Sensory Perception of Sound