CLK3 Protein (CLK3) (GST tag)

Catalog No. ABIN7317780

Product Details

Target: CLK3

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CLK3 protein is labelled with GST tag.

Purpose: Recombinant Human CLK3 Protein (GST Tag)

Sequence: Met 1-Arg 490

Characteristics: A DNA sequence encoding the human CLK3 isoform b (NP_003983.2) (Met 1-Arg 490) was fused with the GST tag at the N-terminus.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM GSH, 0.5 mM PMSF, 25 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CLK3

Alternative Name: CLK3 (CLK3 Products)

Synonyms: PHCLK3 Protein, PHCLK3/152 Protein, AI256811 Protein, CDC like kinase 3 Protein, CDC-like kinase 3 Protein, CLK3 Protein, Clk3 Protein

Background:

Background: Dual specificity protein kinase CLK3, also known as CDC-like kinase 3, and CLK3, is a member of CMGC Ser/Thr protein kinase family and Lammer subfamily. Mammalian CLK is the prototype for a family of dual specificity kinases (termed Lammer kinases) that have been conserved in evolution. CLK family members have shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. The three members of the CLK family of kinases (CLK1, CLK2, and CLK3) have been shown to undergo conserved alternative splicing to generate catalytically active and inactive isoforms. The human CLK2 and CLK3 are found within the nucleus and display dual-specificity kinase activity. The truncated isoforms, hCLK2(T) and hCLK3(T), colocalize with SR proteins in nuclear speckles. CLK3 may play a role in the development and progression of azoospermia.

Synonym: PHCLK3,PHCLK3/152

Molecular Weight: 85 kDa

NCBI Accession: NP_003983