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CD97 Protein (CD97) (His tag)

CD97 Origin: Human Host: HEK-293 Cells Recombinant > 85 % as determined by reducing SDS-PAGE. Active
Catalog No. ABIN7317595
  • Target See all CD97 Proteins
    CD97
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 7
    • 2
    • 1
    Human
    Source
    • 4
    • 3
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CD97 protein is labelled with His tag.
    Purpose
    Recombinant Human CD97 Protein (His Tag)(Active)
    Sequence
    Met 1-Gln 398
    Characteristics
    A DNA sequence encoding the first 398 amino acids (Met 1-Gln 398) of human CD97 isoform 2 (NP_001775.2) extracellular domain was fused with a polyhistidine tag at the C-terminus.
    Purity
    > 85 % as determined by reducing SDS-PAGE.
    Endotoxin Level
    < 1.0 EU per μg as determined by the LAL method.
    Biological Activity Comment
    Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD97 at 10 μg/ml (100 μl/well) can bind recombinant human CD55 at a linear range of 0.46-30 μg/ml.
    Top Product
    Discover our top product CD97 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile PBS, pH 7.4
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    CD97
    Alternative Name
    CD97 (CD97 Products)
    Synonyms
    AA409984 Protein, TM7LN1 Protein, adhesion G protein-coupled receptor E5 Protein, Adgre5 Protein, ADGRE5 Protein
    Background

    Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium binding site. Muture CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isforms that are related to the calium binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.

    Synonym: CD97,TM7LN1

    Molecular Weight
    42.6 kDa
    NCBI Accession
    NP_001775
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