CD6 Protein (CD6) (His tag)
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- Target See all CD6 Proteins
- CD6
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Rat
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD6 protein is labelled with His tag.
- Purpose
- Recombinant Rat CD6/TP120 Protein (His Tag)(Active)
- Sequence
- Met1-Asn396
- Characteristics
- A DNA sequence encoding the rat CD6 (Q812A4) (Met1-Asn396) was expressed with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity Comment
- 1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3 , The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to ratCD6-His coated plates (10μg/mL, 100 μL/well), approximately 39.2% will adhere after 60 minutes at 37°C.
- Top Product
- Discover our top product CD6 Protein
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CD6 (CD6) (AA 18-402) protein (His tag)
CD6 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD6 (CD6) protein (His tag)CD6 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
CD6 (CD6) (AA 18-398) protein (His tag)CD6 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD6 (CD6) (AA 308-400) protein (GST tag)CD6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD6 (CD6) (Active) protein (Fc Tag)CD6 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
CD6 (CD6) (Active) protein (His tag)CD6 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
CD6 (CD6) (Active) protein (Fc Tag)CD6 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CD6
- Alternative Name
- CD6/TP120 (CD6 Products)
- Synonyms
- TP120 Protein, OX52 Protein, CD6 antigen Protein, CD6 molecule Protein, Cd6 molecule Protein, Cd6 Protein, CD6 Protein
- Background
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Background: T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.
Synonym: CD6
- Molecular Weight
- 42.5 kDa
- UniProt
- Q812A4
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