CD6 Protein (CD6) (His tag)

Catalog No. ABIN7194910

Product Details

Target: CD6

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CD6 protein is labelled with His tag.

Purpose: Recombinant Mouse CD6/TP120 Protein (His Tag)(Active)

Sequence: Met 1-Gly396

Characteristics: A DNA sequence encoding the extracellular domain of mouse CD6 (Q91WN5) (Met 1-Gly396) was expressed with a C-terminal polyhistidine tag.

Purity: > 90 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to mCD6-His coated plates (5μg/mL, 100 μL/well) , approximately more than 15% of cells will adhere after 60 minutes at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD6

Alternative Name: CD6/TP120 (CD6 Products)

Synonyms: TP120 Protein, OX52 Protein, CD6 antigen Protein, CD6 molecule Protein, Cd6 molecule Protein, Cd6 Protein, CD6 Protein

Background:

Background: T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

Synonym: CD6 antigen; Cd6

Molecular Weight: 42.6 kDa

UniProt: Q91WN5