CD59 Protein (CD59) (His tag)

Catalog No. ABIN7321145

Product Details

Target: CD59

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This CD59 protein is labelled with His tag.

Purpose: Recombinant Rat CD59 Protein (His Tag)

Sequence: Met1-Asn100

Characteristics: A DNA sequence encoding the rat CD59 (P27274) (Met1-Asn100) was expressed, fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD59

Alternative Name: CD59 (CD59 Products)

Synonyms: 16.3A5 Protein, 1F5 Protein, EJ16 Protein, EJ30 Protein, EL32 Protein, G344 Protein, HRF-20 Protein, HRF20 Protein, MAC-IP Protein, MACIF Protein, MEM43 Protein, MIC11 Protein, MIN1 Protein, MIN2 Protein, MIN3 Protein, MIRL Protein, MSK21 Protein, p18-20 Protein, Cd59a Protein, Cd59b Protein, MACIP Protein, CD59 molecule (CD59 blood group) Protein, CD59 molecule Protein, CD59 Protein, Cd59 Protein

Background:

Background: CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Synonym: CD59

Molecular Weight: 10.3 kDa

UniProt: P27274

Pathways: Complement System