CD28 Protein (CD28) (His tag)

Catalog No. ABIN7319757

Product Details

Target: CD28

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Cynomolgus

Source: Human Cells

Purification tag / Conjugate: This CD28 protein is labelled with His tag.

Purpose: Recombinant Human/Cynomolgus CD28/TP44 (N-His)

Sequence: Asn19-Pro152

Characteristics: Recombinant Human T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1 IEU/μg as determined by LAL test.

Biological Activity Comment: Immobilized  Human/Cynomolgus CD28-His(Cat#C406) at 2μg/ml (100 μl/well) can bind Anti-CD28 mAb(Cat#A063). The ED50 of  Anti-CD28 mAb(Cat#A063) is 15.3 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD28

Alternative Name: / CD28/TP44 (CD28 Products)

Synonyms: Tp44 Protein, CD28RNA Protein, cd28 Protein, CD28 molecule Protein, CD28 antigen Protein, Cd28 molecule Protein, CD28 molecule L homeolog Protein, CD28 Protein, Cd28 Protein, cd28.L Protein

Background:

Background: T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Synonym: CD28, CD28 antigen, CD28 Molecule, T-cell-specific surface glycoprotein CD28, Tp44, TP44

Molecular Weight: 16.7 kDa

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response