CD200 Protein (CD200) (His tag)

Catalog No. ABIN7320017

Product Details

Target: CD200

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CD200 protein is labelled with His tag.

Purpose: Recombinant Mouse CD200 Protein (His Tag)(Active)

Sequence: Met 1-Gly 232

Characteristics: A DNA sequence encoding the extracellular domain of mouse CD200 (O54901.1) (Met 1-Gly 232) was expressed with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 at 1 μg/ml (100ul/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD200

Alternative Name: CD200 (CD200 Products)

Synonyms: MOX1 Protein, MOX2 Protein, MRC Protein, OX-2 Protein, CD200 Protein, Cspmo2 Protein, MRCOX2 Protein, Mox2 Protein, DKFZp459C0716 Protein, OX2 Protein, CD200 molecule Protein, Cd200 molecule Protein, CD200 antigen Protein, CD200 Protein, Cd200 Protein

Background:

Background: CD200 (OX-2) is a cell surface glycoprotein that imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. Signals delivered through the CD200:CD200R axis have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. The role of CD200-CD200R signaling in immune regulation of the central nervous system has become a popular field of research in recent years. Many studies have shown that there is a close correlation between CD200-CD200R, microglia activation, and Parkinson's disease (PD). The ability of CD200 to suppress myeloid cell activation is critical for maintaining normal tissue homeostasis but may also enhance the survival of migratory neoplastic cells. CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 has been characterized as an important immunoregulatory molecule, increased expression of which can lead to decreased transplant rejection, autoimmunity, and allergic disease. Elevated CD200 expression has been reported to be associated with poor prognosis in a number of human malignancies. In addition, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: Mox2;OX2

Molecular Weight: 24 kDa

UniProt: O54901