CA10 Protein (His tag)
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- Target See all CA10 Proteins
- CA10 (Carbonic Anhydrase X (CA10))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CA10 protein is labelled with His tag.
- Purpose
- Recombinant Human Carbonic Anhydrase 10/CA10 Protein (His Tag)(Active)
- Sequence
- Met 1-Asn 300
- Characteristics
- A DNA sequence encoding the human CARPX (NP_001076002.1) (Met 1-Asn 300) was expressed with a C-terminal polyhistidine tag.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its esterase activity. The specific activity is >10 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
- Top Product
- Discover our top product CA10 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CA10 (Carbonic Anhydrase X (CA10))
- Alternative Name
- Carbonic Anhydrase 10/CA10 (CA10 Products)
- Synonyms
- CA-RPX Protein, CARPX Protein, HUCEP-15 Protein, 2700029L05Rik Protein, BB085816 Protein, Ca10 Protein, CA10 Protein, si:ch211-29a20.1 Protein, zgc:123167 Protein, carbonic anhydrase 10 Protein, carbonic anhydrase X L homeolog Protein, carbonic anhydrase Xa Protein, CA10 Protein, Car10 Protein, ca10.L Protein, Ca10 Protein, ca10a Protein
- Background
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Background: Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
Synonym: Carbonic Anhydrase-Related Protein 10, Carbonic Anhydrase-Related Protein X, CA-RP X, CARP X, Cerebral Protein 15, CA10,CA-RPX,CARPX,HUCEP-15
- Molecular Weight
- 33 kDa
- NCBI Accession
- NP_001076002
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