CA12 Protein (His tag)
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- Target See all CA12 Proteins
- CA12 (Carbonic Anhydrase 12 (CA12))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CA12 protein is labelled with His tag.
- Purpose
- Recombinant Mouse Carbonic Anhydrase XII/CA12 Protein (His Tag)(Active)
- Sequence
- Met 1-Ser 301
- Characteristics
- A DNA sequence encoding the extracellular domain (Met 1-Ser 301) of mouse CA12 (NP_848483.2) precursor was expressed with a C-terminal polyhistidine tag.
- Purity
- > 95 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its esterase activity. The specific activity is >50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.4 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
- Top Product
- Discover our top product CA12 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CA12 (Carbonic Anhydrase 12 (CA12))
- Alternative Name
- Carbonic Anhydrase XII/CA12 (CA12 Products)
- Synonyms
- CAXII Protein, HsT18816 Protein, CAR12 Protein, 2310047E01Rik Protein, AI314958 Protein, Ca12 Protein, ca12 Protein, carbonic anhydrase 12 Protein, carbonic anhydrase XII L homeolog Protein, CA12 Protein, Car12 Protein, ca12.L Protein
- Background
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Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
Synonym: Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; CA12; Carbonate dehydratase XII; CAXII;Car12
- Molecular Weight
- 32.8 kDa
- NCBI Accession
- NP_848483
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