CHST15 Protein (His tag)
-
- Target See all CHST15 Proteins
- CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CHST15 protein is labelled with His tag.
- Purpose
- Recombinant Human CHST15/BRAG Protein (His Tag)
- Sequence
- Ser 99-Thr 561
- Characteristics
- A DNA sequence encoding the human BRAG (NP_056976.2) extracellular domain (Ser 99-Thr 561) was expressed, with a polyhistidine tag at the N-terminus.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product CHST15 Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))
- Alternative Name
- CHST15/BRAG (CHST15 Products)
- Synonyms
- GALNAC4S-6ST Protein, BRAG Protein, RP11-47G11.1 Protein, 4631426J05Rik Protein, GalNAcS-6ST Protein, MAd5 Protein, mKIAA0598 Protein, GalNAc4S6ST Protein, Galnac4s-6st Protein, carbohydrate sulfotransferase 15 Protein, carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 Protein, CHST15 Protein, chst15 Protein, Chst15 Protein
- Background
-
Background: Carbohydrate sulfotransferase 15, also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, GalNAc4S-6ST, B-cell RAG-associated gene protein, CHST15 and BRAG, is a single-pass type I I membrane protein which belongs to the sulfotransferase 1 family. CHST15 / BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. CHST15 / BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 / BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression.
Synonym: BRAG;GALNAC4S-6ST
- Molecular Weight
- 56 kDa
- NCBI Accession
- NP_056976
- Pathways
- Glycosaminoglycan Metabolic Process
-