BRD4 Protein

Catalog No. ABIN7319875

Product Details

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human BRD4 (N-10His-Flag)

Sequence: Glu49-Glu460

Characteristics: Recombinant Human Bromodomain-containing protein 4 is produced by our E.coli expression system and the target gene encoding Glu49-Glu460 is expressed with a 10His, Flag tag at the N-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 7.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4 (BRD4 Products)

Synonyms: brd4-b Protein, BRD4 Protein, fb18g03 Protein, wu:fb18g03 Protein, CAP Protein, HUNK1 Protein, HUNKI Protein, MCAP Protein, Brd5 Protein, WI-11513 Protein, bromodomain containing 4 L homeolog Protein, bromodomain containing 4 Protein, bromodomain-containing protein 4 Protein, brd4.L Protein, BRD4 Protein, brd4 Protein, LOC100409351 Protein, Brd4 Protein

Background:

Background: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.

Synonym: HUNK1, HUNK1bromodomain-containing protein 4, HUNKI, MCAP

Molecular Weight: 49 kDa

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome