BDNF Protein
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- Target See all BDNF Proteins
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human BDNF Protein (with pro peptide)(Active)
- Sequence
- Ala19-Arg247
- Characteristics
- Recombinant Human BDNF is produced by our E.coli expression system and the target gene encoding Ala19-Arg247 is expressed.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Immobilized Human pro-BDNF at 2ug/ml(100 μl/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human pro-BDNF is 1.4 ug/ml .
- Top Product
- Discover our top product BDNF Protein
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Brain-Derived Neurotrophic Factor (BDNF) (AA 1-255) protein (GST tag)
BDNF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) protein (His-IF2DI Tag)BDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) protein (GST tag)BDNF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Brain-Derived Neurotrophic Factor (BDNF) (AA 137-237), (partial) protein (GST tag)BDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % SDS, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- Alternative Name
- BDNF (BDNF Products)
- Synonyms
- ANON2 Protein, BULN2 Protein, bdnf-A Protein, BDNF Protein, bdnf Protein, brain derived neurotrophic factor Protein, brain-derived neurotrophic factor Protein, brain-derived neurotrophic factor L homeolog Protein, brain-derived neurotrophic factor S homeolog Protein, BDNF Protein, Bdnf Protein, bdnf Protein, bdnf.L Protein, bdnf.S Protein
- Background
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Background: The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
Synonym: Brain-Derived Neurotrophic Factor, BDNF, Abrineurin
- Molecular Weight
- 25.9 kDa
- UniProt
- P23560
- Pathways
- RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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