Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

BTD Protein (His tag)

BTD Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Catalog No. ABIN7194393
  • Target See all BTD Proteins
    BTD (Biotinidase (BTD))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 4
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This BTD protein is labelled with His tag.
    Purpose
    Recombinant Human Biotinidase/BTD Protein (His Tag)(Active)
    Sequence
    Met 1-Asp543
    Characteristics
    A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Endotoxin Level
    < 1.0 EU per μg as determined by the LAL method.
    Biological Activity Comment
    Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmol/min/μg.
    Top Product
    Discover our top product BTD Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile PBS, pH 7.4
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    BTD (Biotinidase (BTD))
    Alternative Name
    Biotinidase/BTD (BTD Products)
    Synonyms
    BTD Protein, CG3599 Protein, CT12113 Protein, Dmel\\CG3599 Protein, zgc:153333 Protein, Biotinase Protein, Biotinidase Protein, biotinidase Protein, Btnd Protein, btd Protein, BTD Protein, Btd Protein
    Background

    Background: Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.

    Synonym: BTD

    Molecular Weight
    58.2 kDa
    UniProt
    P43251
You are here:
Support