BFAR Protein

Catalog No. ABIN7321012

Product Details

Target: BFAR (Bifunctional Apoptosis Regulator (BFAR))

Protein Type: Recombinant

Origin: Streptomyces hygroscopicus

Source: Escherichia coli (E. coli)

Purpose: Recombinant Streptomyces hygroscopicus Bar Protein

Sequence: Met1-Ile183

Characteristics: Recombinant Streptomyces hygroscopicus Bar is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: BFAR (Bifunctional Apoptosis Regulator (BFAR))

Alternative Name: Bar (BFAR Products)

Background:

Background: Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

Synonym: Phosphinothricin N-acetyltransferase, PPT N-acetyltransferase, Phosphinothricin-resistance protein, bar

Molecular Weight: 20.6 kDa

UniProt: P16426