ENPP7 Protein (His tag)
-
- Target See all ENPP7 Proteins
- ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))
- Protein Type
- Recombinant
-
Origin
- Mouse
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ENPP7 protein is labelled with His tag.
- Purpose
- Recombinant Mouse ENPP7/NPP-7 Protein (His Tag)
- Sequence
- Met 1-Gln 421
- Characteristics
- A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a polyhistidine tag at the C-terminus.
- Purity
- > 90 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product ENPP7 Protein
-
Alkaline Sphingomyelinase (Alk-Smase) (AA 1-458) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant WB, AA, AP, ELISA
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.6
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))
- Alternative Name
- ENPP7/NPP-7 (ENPP7 Products)
- Synonyms
- ALK-SMase Protein, E-NPP 7 Protein, NPP-7 Protein, Alk-SMase Protein, Gm254 Protein, ectonucleotide pyrophosphatase/phosphodiesterase 7 Protein, ENPP7 Protein, Enpp7 Protein
- Background
-
Background: Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
Synonym: Alk-SMase;Gm254
- Molecular Weight
- 47 kDa
- UniProt
- Q3TIW9
-
