ENPP7 Protein (His tag)

Catalog No. ABIN7194214

Product Details

Target: ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ENPP7 protein is labelled with His tag.

Purpose: Recombinant Human ENPP7/NPP-7 Protein (His Tag)

Sequence: Met 1-Ser 439

Characteristics: A DNA sequence encoding the human ENPP7 (NP_848638.2) (Met 1-Ser 439) was fused with a C-terminal polyhistidine tag.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ENPP7 (Alkaline Sphingomyelinase (Alk-Smase))

Alternative Name: ENPP7/NPP-7 (ENPP7 Products)

Synonyms: ALK-SMase Protein, E-NPP 7 Protein, NPP-7 Protein, Alk-SMase Protein, Gm254 Protein, ectonucleotide pyrophosphatase/phosphodiesterase 7 Protein, ENPP7 Protein, Enpp7 Protein

Background:

Background: Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.

Synonym: ALK-SMase;E-NPP7;NPP-7;NPP7

Molecular Weight: 49 kDa

NCBI Accession: NP_848638