AK4 Protein

Catalog No. ABIN7194161

Product Details

Target: AK4 (Adenylate Kinase 4 (AK4))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purpose: Recombinant Human AK4/AK3L1 Protein

Sequence: Ala 2-Tyr 223

Characteristics: A DNA sequence encoding the human AK4 (P27144-1) (Ala 2-Tyr 223) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: AK4 (Adenylate Kinase 4 (AK4))

Alternative Name: AK4/AK3L1 (AK4 Products)

Synonyms: AK 4 Protein, AK3 Protein, AK3L1 Protein, AK3L2 Protein, ak3l1 Protein, wu:fc37g02 Protein, zgc:85790 Protein, AK4 Protein, Ak-3 Protein, Ak-4 Protein, Ak3 Protein, Ak3l1 Protein, D4Ertd274e Protein, Ak3l2 Protein, adenylate kinase 4 Protein, AK4 Protein, ak4 Protein, Ak4 Protein, ADK4 Protein

Background:

Background: Adenylate kinase isoenzyme 4; mitochondrial; also known as ATP-AMP transphosphorylase; Adenylate kinase 3-like; AK4 and AK3L1; is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

Synonym: AK3;AK3L1;AK3L2;AK4

Molecular Weight: 25.3 kDa

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process