CD73 Protein (His tag)
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- Target See all CD73 (NT5E) Proteins
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Rat
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD73 protein is labelled with His tag.
- Purpose
- Recombinant Rat CD73/NT5E Protein (His Tag)(Active)
- Sequence
- Met1-Lys549
- Characteristics
- A DNA sequence encoding the rat NT5E(Q66HL0) (Met1-Lys549) was expressed with a polyhistidine tag at the C-terminus.
- Purity
- > 90 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity Comment
- Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (R&D Systems, Catalog # DY996).The specific activity is >15,000 pmol/min/μg.
- Top Product
- Discover our top product NT5E Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Alternative Name
- CD73/NT5E (NT5E Products)
- Synonyms
- CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein
- Background
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Background: 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
Synonym: CD73; E5NT;Nucleotidase; NT5E;ecto-5'-nucleotidase;5'-Nucleotidase
- Molecular Weight
- 59.4 kDa
- UniProt
- Q66HL0
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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