PDPK1 Protein (His tag)

Catalog No. ABIN7194071

Product Details

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This PDPK1 protein is labelled with His tag.

Purpose: Recombinant Human PDK-1 Protein (His Tag)

Sequence: Ser 29-Ala 436

Characteristics: A DNA sequence encoding the mature form of human PDK1 (NP_002601.1) (Ser 29-Ala 436) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.5, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK-1 (PDPK1 Products)

Synonyms: pdpk1 Protein, MGC82080 Protein, PDK1 Protein, PDPK2 Protein, PRO0461 Protein, Pdk1 Protein, zgc:153787 Protein, 3'-phosphoinositide-dependent protein kinase 1 Protein, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Protein, ATPDK1 Protein, AtPDK1 Protein, T32M21.110 Protein, T32M21_110 Protein, zgc:77318 Protein, 3-phosphoinositide dependent protein kinase 1 Protein, 3-phosphoinositide dependent protein kinase 1 L homeolog Protein, 3-phosphoinositide dependent protein kinase-1 Protein, 3-phosphoinositide dependent protein kinase 1a Protein, 3-phosphoinositide-dependent protein kinase 1 Protein, 3'-phosphoinositide-dependent protein kinase 1 Protein, 3-phosphoinositide dependent protein kinase 1b Protein, PDPK1 Protein, pdpk1.L Protein, pdpk1 Protein, Pdpk1 Protein, pdpk1a Protein, LOC100380750 Protein, pdk-1 Protein, PDK1 Protein, pdpk1b Protein

Background:

Background: Pyruvate dehydrogenase kinase, isozyme 1, also known as [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial and PDK1, is a member of the PDK / BCKDK protein kinase family. PDK-1 is expressed predominantly in the heart. It contains one histidine kinase domain. Pyruvate dehydrogenase kinase (PDK) isoforms are molecular switches that downregulate the pyruvate dehydrogenase complex (PDC) by reversible phosphorylation in mitochondria. An inhibitory effect of lipoic acid on PDKs would result in less phosphorylation of E1 and hence increased PDC activity. At least two isoenzymic forms of pyruvate dehydrogenase kinase ( PDK-1 and PDK-2 ) may be involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase complex by phosphorylation. PDK-3 appears to have the highest specific activity among the three isoenzymes. PDK-1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Synonym: PDK1

Molecular Weight: 48.6 kDa

NCBI Accession: NP_002601

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals