YWHAE Protein

Catalog No. ABIN7194064

Product Details

Target: YWHAE (14-3-3 epsilon (YWHAE))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human 14-3-3 epsilon/YWHAE Protein

Sequence: Met 1-Gln 255

Characteristics: A DNA sequence encoding the human YWHAE (NP_006752.1) (Met 1-Gln 255) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus.

Purity: > 96 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 0.25 mM DTT, 25 % glycerol, 0.5 mM GSH, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: YWHAE (14-3-3 epsilon (YWHAE))

Alternative Name: 14-3-3 epsilon/YWHAE (YWHAE Products)

Synonyms: 14-3-3 Protein, 14-3-3 epsilon Protein, 14-3-3-e Protein, 14-3-3-epsilon Protein, 14-3-30epsilon Protein, 14-3-3EPSILON Protein, 14-3-3e Protein, 14-3-3omicron Protein, 1433epsilon Protein, 143E_DROME Protein, CG31196 Protein, CT24092 Protein, D14-3-3e Protein, D14-3-3epsilon Protein, Dmel\\CG31196 Protein, EK3-5 Protein, PAR-5 Protein, PAR5 Protein, Par-5 Protein, SR3-9 Protein, Su(Raf)3B Protein, anon-WO0172774.141 Protein, anon-WO02059370.52 Protein, d14-3-3epsilon Protein, eps Protein, l(3)j2B10 Protein, par-5 Protein, 14-3-3epsilon Protein, kcip-1 Protein, mdcr Protein, mds Protein, LOC581376 Protein, Ywhae Protein, ACYPI002517 Protein, YWHAE Protein, 14-3-3E Protein, KCIP-1 Protein, MDCR Protein, MDS Protein, AU019196 Protein, fe38c12 Protein, wu:fb48f06 Protein, wu:fe38c12 Protein, wu:fi22e10 Protein, ywhae Protein, zgc:55377 Protein, zgc:77062 Protein, CG31196 gene product from transcript CG31196-RD Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon Protein, 14-3-3 epsilon Protein, 14-3-3 epsilon protein Protein, 14-3-3 protein epsilon Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon S homeolog Protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 Protein, 14-3-3epsilon Protein, ywhae Protein, LOC581376 Protein, Ywhae Protein, HCAG_04173 Protein, PTRG_02836 Protein, PTRG_04459 Protein, UREG_07864 Protein, YWHAE Protein, ywhae.S Protein, ywhae1 Protein

Background:

Background: YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

Synonym: 14-3-3E,HEL2,KCIP-1,MDCR,MDS

Molecular Weight: 29.4 kDa

NCBI Accession: NP_006752

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase