MPXV E8L protein (His tag)

Catalog No. ABIN7126002

Product Details

Target: MPXV E8L

Protein Type: Recombinant

Origin: Monkeypox Virus

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Purpose: Monkeypox virus (strain Zaire-96-I-16) E8L Protein, His Tag

Sequence: Met 1 - Thr 275

Characteristics: Monkeypox virus (strain Zaire-96-I-16) E8L, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 275 (Accession # Q8V4Y0-1).

Purity: >90% as determined by SDS-PAGE.

Endotoxin Level: Less than 0.01 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Storage: -20 °C

Target Details

Target: MPXV E8L

Background: Synonyms: E8L,
Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa , multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. E8L can Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.

Molecular Weight: 33.7 kDa